Iso 8655-6 pdf free download

ISO , was written specifically to define the requirements necessary to produce accurate and reliable calibrations of piston pipettes and other closely related measurement equipment. It details the required methods, test conditions, test equipment, reporting requirements and includes requirements for reporting the required measurement uncertainty values. Today, it is the most critical ISO standard for calibrating piston-operated pipettes, burettes, diluters, and dispensers.

Nor are the guidelines specific to any device; they apply equally well to a broad range of equipment requiring calibration, including not just pipettes, but also medical devices, communication equipment, electronic devices, weight scales, pressure meters, calipers, and more. ISO , on the other hand, is a very detailed set of standards, methods and controls that a pipette calibration provider must follow to correctly and accurately calibrate piston pipettes.

This difference explains how traveling or onsite pipette calibration providers can be accredited to ISO but not to ISO Adherence to the ISO standard covers air displacement pipettes and positive displacement pipettes, single channel and multichannel pipettes both fixed and adjustable volume as well as both manual pipettes and automated pipettes, often called motorized pipettes or electronic pipettes.

ISO accredited laboratories like Pipettes. The standard characterizes both the maximum permissible systematic error, as well as the maximum permissible random error limits for a device at specific volumes ranging from ,uL.

These errors are doubled for multichannel pipettes. ISO states that the primary methodology for measurement of piston-operated pipettes and related measurement apparatus is through gravimetric measurement analysis. Included are the minimum balance requirements at each test volume, important for reporting measurement accuracy at the appropriate resolution. Pipette measurements face the same challenge for many providers.

This standard also requires control not just monitoring of test conditions, along with minimum data points for a valid representation of device performance and measurement analysis. To be considered valid by ISO , pipette measurements must occur in a strictly controlled, vibration-free test environment. The acceptable ranges of environmental conditions are defined as:. The ISO standard defines a step-wise procedure for generating valid, reliable measurement data.

Procedures include proper technique for tip installation, pre-wetting, aspiration and dispensing technique, measurement container requirements, required number of measurements, evaporation rate determination and time lapse for test completion. At the start and at the end of the weighing procedure, the temperature of the test liquid in its container shall be. The barometric pressure in the test room shall be recorded to the nearest 1 kPa and.

The remainder. Record the. If the. NOTE Temperature and barometric pressure are necessary for the choice of the correction factor Z see 8.

Release the operating button slowly, if hand operated, and withdraw the pipette vertically and carefully from the. Touch the delivery orifice against the side wall of the container with the test liquid. Start the timing device this may be omitted if using a weighing vessel with lid. Deliver the contents of the pipette into the weighing vessel, touching. Where applicable, use the blow-out feature of the piston pipette to expel the last drop of liquid before drawing the.

If it is necessary to remove the weighing vessel from the balance pan to permit delivery of the dispensed volume,. Return the weighing vessel to. If the weighing vessel was removed from the balance pan to enable delivery, leave it on the pan for half the time in. If the test volume is or if a weighing vessel with lid is used, omit steps 7.

Multi-channel piston pipettes are similar to single-channel in that they comprise a set of single-volume measuring. For the purposes of the test, each. Fill all channels of the multi-channel pipette by aspirating the test liquid.

Expel only the test liquid aspirated by the. Piston pipettes without an air interface shall be tested in accordance with 7. However, the five-fold prewetting of the. Only change the pipette tips when testing positive-displacement pipettes of type D2 see ISO Wipe the pipette tip on the water container wall after aspiration of the test liquid and prior to expelling it into the. Without removing liquid from the inside of the.

Follow the supplier's. Carry out the testing by delivery into the weighing vessel see 7. Carefully clean the weighing vessel and add a. Place the weighing vessel and its test liquid in the balance case.

Then place the. Leave both for. Measure the mass of the weighing vessel and its test liquid and consider this value as the tare mass prior to the first. Load the piston burette, bubble free, with test liquid from the reservoir, in accordance with the supplier's instructions. Deliver the test liquid from the burette into the weighing vessel, until the selected volume is reached. If the burette is.

When testing partial volumes see 7. Ensure that the upper volume limit of the piston and thus the. When testing piston burettes — especially in the case of automated tests — wiping of the delivery jet on the vessel.

In such cases, ascertain that the weighing. ISO sets out principles for the design of volumetric instruments manufactured from glass or from plastics in order to facilitate the most reliable and convenient use to the intended degree of accuracy.

ISO specifies requirements for an internationally acceptable series of glass beakers for laboratory use. ISO provides methods for the testing, calibration and use of volumetric instruments made from glass in order to obtain the best accuracy in use. ISO contains supplementary information.

The procedu TC - Modification in Subclause 8. TC - Modifications in Foreword and in Clauses 1, 5, 7 and 8. TC - Modifications in Foreword and Clauses 1, 5, 7 and 8. TC - Modifications in Clause 2 and Subclause 6. TC - Modifications in Clauses 2, 3, 6, C. ISO specifies metrological and constructional requirements for volumetric pipettes with one mark total delivery and for volumetric pipettes with two marks, both of which are adequate for general laboratory purposes. The details specified are in conformity with the principles of design and construction of volumetric glassware given in ISO ISO specifies metrological and constructional requirements for graduated pipettes, adequate for general laboratory purposes.

ISO specifies the photometric and titrimetric determination of errors of measurement of piston-operated volumetric apparatus. The tests are applicable to complete systems comprising the basic apparatus and all parts selected for use with the apparatus, disposable or reusable, involved in the measurement by delivery process. These non-gravimetric test methods can be applied: as aids to quality assurance by the supplier; as routine quality assurance and routine calibrations by the use ISO specifies dimensions, material and constructional and metrological requirements of graduated measuring cylinders of tall form Type 1a and Type 1b and of squat form Type 2.

All types are suitable for general laboratory use. The specifications in ISO are in conformity with the principles of design and construction of volumetric glassware given in ISO This International Standard provides metrological and construction requirements for an internationally acceptable series of burettes, suitable for general laboratory purposes. The details specified are in accordance with the principles of design and construction of volumetric glassware given in ISO For piston burettes, see ISO ISO specifies metrological requirements, maximum permissible errors, requirements for marking and information to be provided for users for dispensers.

It is applicable to dispensers with nominal volumes from 1 microlitre up to millilitres, designed to deliver their volume Ex. ISO specifies metrological requirements, maximum permissible errors, requirements for marking and information to be provided for users, for dilutors with a sample uptake capacity In from 5 microlitres to 10 millilitres and a diluent capacity Ex from 50 microlitres to millilitres.

They are designed to deliver the sample and diluent together in measured proportion and measured volume. ISO specifies the general requirements for piston-operated volumetric apparatus. It is applicable to piston pipettes, piston burettes, dilutors and dispensers. It furthermore defines terms for the use of piston-operated volumetric apparatus and gives a list of equivalent terms.

It also gives user recommendations. ISO is not applicable to medical products intended for use on human beings, e. Your shopping cart is empty! BACK Sep Abstract German French Slovenian Piston-operated volumetric apparatus - Part 6: Gravimetric methods for the determination of measurement error ISO ISO specifies the gravimetric determination of errors of measurement for conformity testing of piston-operated volumetric apparatus.

Publication Date. Technical Committee. Drafting Committee. Current Stage. Completion Date. Ref Project.

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ISO consists of the following parts, under the general title Piston-operated volumetric apparatus: — Part 1: Terminology, general requirements and user recommendations — Part 2: Piston pipettes — Part 3: Piston burettes — Part 4: Dilutors — Part 5: Dispensers — Part 6: Gravimetric methods for the determination of measurement error The following part is under preparation: — Part 7: Non-gravimetric methods for the determination of measurement error Annex A forms a normative part of this part of ISO Annex B is for information only.

The tests specified should be carried out by trained personnel. For the metrological requirements, maximum permissible errors, requirements for marking and information to be provided for users for piston-operated volumetric apparatus, see ISO for piston pipettes, see ISO for piston burettes, see ISO for dilutors and see ISO for dispensers.

Alternative test methods such as photometric and titrimetric methods will be the subject of a future Part 7 to ISO For dated references, subsequent amendments to, or revisions of, any of these publications do not apply.

However, parties to agreements based on this part of ISO are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below.